Description
Mouse monoclonal antibody raised against a partial recombinant SIRT1, clone 7B7.

Size
100 µg

Purification
Protein A purified

Immunogen
SIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (37 KDa).

 

Note: This item was formerly catalog no. ABB-4012 (ABB4012)

Applications

Western Blot	SIRT1 Monoclonal Antibody [7B7]. Western Blot analysis of SIRT1 expression in HeLa NE.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged SIRT1 is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of Monoclonal Antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall.
Immunofluorescence	Immunofluorescence of monoclonal antibody to SIRT1 on HeLa cell.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.