Description
Mouse monoclonal antibody raised against a full length recombinant SAE1, clone 1G4-1G5.

Size
100 µg

Purification
Protein A purified

Immunogen
SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Lambda

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (64.17 KDa).

 

Note: This item was formerly catalog no. ABB-8088 (ABB8088)

Applications

Western Blot	SAE1 Monoclonal Antibody, clone 1G4-1G5. Western Blot analysis of SAE1 expression in HeLa.
Immunoprecipitation	Not Determined
ELISA	Detection limit for recombinant GST tagged SAE1 is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to SAE1 on formalin-fixed paraffin-embedded human adrenal gland. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.