QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation

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QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation

Base Product #: P-5005 Availability: Usually Ships In 1-2 Days

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Catalog No.+	Product Name	Select Qty.	Price
P-5005-1433A-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - 14-3-3-α (96 assays)		$469.00
P-5005-1433B-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - 14-3-3-β (96 assays)		$469.00
P-5005-1433G-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - 14-3-3-γ (96 assays)		$469.00
P-5005-APC-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - APC (96 assays)		$469.00
P-5005-ATRIP-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - ATRIP (96 assays)		$469.00
P-5005-AURA-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Aurora A (96 assays)		$469.00
P-5005-AURB-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Aurora B (96 assays)		$469.00
P-5005-BIN1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Bin 1 (96 assays)		$469.00
P-5005-BMI1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Bmi 1 (96 assays)		$469.00
P-5005-CAK1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CAK1 (96 assays)		$469.00
P-5005-CDC13-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC13 (96 assays)		$469.00
P-5005-CDC25A-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC25A (96 assays)		$469.00
P-5005-CDC25B-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC25B (96 assays)		$469.00
P-5005-CDC25C-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC25C (96 assays)		$469.00
P-5005-CDC27-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC27 (96 assays)		$469.00
P-5005-CDC2P34-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC2P34 (96 assays)		$469.00
P-5005-CDC37-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC37 (96 assays)		$469.00
P-5005-CDC42-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC42 (96 assays)		$469.00
P-5005-CDC6-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDC6 (96 assays)		$469.00
P-5005-CDK2-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDK2 (96 assays)		$469.00
P-5005-CDK6-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDK6 (96 assays)		$469.00
P-5005-CDK7-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDK7 (96 assays)		$469.00
P-5005-CDK8-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDK8 (96 assays)		$469.00
P-5005-CDK9-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CDK9 (96 assays)		$469.00
P-5005-CHFR-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - CHFR (96 assays)		$469.00
P-5005-CHK1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Chk 1 (96 assays)		$469.00
P-5005-CHK2-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Chk 2 (96 assays)		$469.00
P-5005-CYCLA-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin A (96 assays)		$469.00
P-5005-CYCLB-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin B (96 assays)		$469.00
P-5005-CYCLB2-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin B2 (96 assays)		$469.00
P-5005-CYCLD1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin D1 (96 assays)		$469.00
P-5005-CYCLD2-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin D2 (96 assays)		$469.00
P-5005-CYCLD3-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin D3 (96 assays)		$469.00
P-5005-CYCLE-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin E (96 assays)		$469.00
P-5005-CYCLE2-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin E2 (96 assays)		$469.00
P-5005-CYCLG1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin G1 (96 assays)		$469.00
P-5005-CYCLH-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - Cyclin H (96 assays)		$469.00
P-5005-DHFR-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - DHFR (96 assays)		$469.00
P-5005-GADD153-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - GADD153 (96 assays)		$469.00
P-5005-GADD45-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - GADD45 (96 assays)		$469.00
P-5005-P14-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P14 (96 assays)		$469.00
P-5005-P15-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P15 (96 assays)		$469.00
P-5005-P16-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P16 (96 assays)		$469.00
P-5005-P18-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P18 (96 assays)		$469.00
P-5005-P19-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P19 (96 assays)		$469.00
P-5005-P21-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P21 (96 assays)		$469.00
P-5005-P27-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P27 (96 assays)		$469.00
P-5005-P57-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P57 (96 assays)		$469.00
P-5005-P73-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - P73 (96 assays)		$469.00
P-5005-PLK1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - PLK1 (96 assays)		$469.00
P-5005-PTEN-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - PTEN (96 assays)		$469.00
P-5005-SHP1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - SHP1 (96 assays)		$469.00
P-5005-SKP1-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - SKP1 (96 assays)		$469.00
P-5005-SKP2-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - SKP2 (96 assays)		$469.00
P-5005-TK-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - TK (96 assays)		$469.00
P-5005-TS-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - TS (96 assays)		$469.00

Product Overview
Product Details
Product Components
User Guide & MSDS

Product Overview

The QuantiSir™ Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation series, offering a flexible choice of different kits used for measuring the knockdown of 56 common genes related to cell cycle regulation, is part of Epigentek's advanced gene knockdown assay system to quantify gene knockdown induced by siRNA or antisense oligonucleotide at the protein level in cultured cells or tissues. The kit offers a more highly convenient process than traditional methods such as Northern blot, quantitative RT-PCR, and Western Blot. The kit addresses and corrects traditional problems such as the decrease in the amount of a specific mRNA not typically correlating well with protein levels present in the cell, and the lack of ability to discriminate between samples in which the differences in protein levels are minimal.

WHY CHOOSE THE QUANTISIR™ SPECIFIC GENE KNOCKDOWN QUANTIFICATION KIT FOR CELL CYCLE REGULATION?

Quick and efficient procedure -- completion of entire assay requires only 4 hours.
Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
The internal control is included in the kits to correct for variations for the cell number or protein concentrations.
Strip microwell format makes the assay flexible: manual or high throughput.
Simple, reliable, and consistent assay conditions.

Product Details

The QuantiSir™ Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation is specifically designed for quantifying gene knockdown induced by siRNA or antisense oligonucleotide at the protein level in the cultured cells or tissues. In the assay, the cell lysates containing the targeted protein are stably spotted on the specifically treated microwells with a unique protein capture buffer. The spotted protein can then be recognized with the target-specific antibody and colorimetrically measured through detection antibody-chromogen reaction system.

SCHEMATIC PROCEDURE:
	Quantification of HDAC1 knockdown. MCF-7 cells were treated or untreated with HDAC1 siRNA. Protein extracts were prepared and used for detection of HDAC1 protein level.

Product Components

Q1 (Extraction Buffer
Q2 (10X Wash Buffer)
Q3 (Protein Capture Buffer)
Q4 (Blocking Buffer)
Q5 (Antibody Buffer)
Q6 (Developing Solution)
Q7 (Stop Solution)
GAPDH Control Antibody*
Capture Antibody*
Detection Antibody*
8-Well Assay strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.