Progerin Monoclonal Antibody [13A4]

Description
Mouse monoclonal antibody to Progerin, Clone 13A4

Formulation
Liquid. Neat cell culture supernatant containing 0.02% sodium azide.

Immunogen
Synthetic KLH-coupled peptide corresponding to aa 604-611 (G604AQSPQNC611) at the C-terminus of human progerin.

Specificity
Human

Isotype
IgG1

Purification
Serum

Storage
Store at +4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.

Alternative Names
Delta50 Lamin A

Application
ICC (1:10), IP (20 µl/assay), WB (1:1000)

Fig. 1.  Western blot analysis (10% PAGE) of whole cell lysate of HeLa cells ectopically expressing FLAG-tagged human progerin. Lane 1: anti-Lamin A/C antibody (N18) (1:2000), Lane 2: anti-FLAG-tag antibody (M2) (1:5000), Lane 3: Progerin Monoclonal Antibody [13A4] (1:5)

Fig. 2.  Immunoprecipitation of progerin from lysates of Hutchinson-Gilford Progerin Monoclonal Antibody [13A4]. The immunoprecipitates were separated by SDS-PAGE, blotted and incubated with Progerin Monoclonal Antibody [13A4] (1:200). The asteriks labels the IgG band.