Description
Mouse monoclonal antibody raised against a full length recombinant PRKRA, clone 1B9-1A7.

Size
100 µg

Purification
Protein A purified

Immunogen
PRKRA (AAH09470, 1 a.a. ~ 314 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (60.54 KDa).

 

Note: This item was formerly catalog no. ABB-5008 (ABB5008)

Applications

Western Blot	PRKRA Monoclonal Antibody, clone 1B9-1A7. Western Blot analysis of PRKRA expression in human liver. PRKRA Monoclonal Antibody, clone 1B9-1A7. Western Blot analysis of PRKRA expression in HeLa. Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA Monoclonal Antibody, clone 1B9-1A7. Lane 1: PRKRA transfected lysate (34.4 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged PRKRA is approximately 0.03 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. [Antibody Concentration: 5 µg/ml] Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. [Antibody Concentration: 5 µg/ml]
Immunofluorescence	Not determined

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.