Description
Mouse monoclonal antibody raised against a partial recombinant PRKCA, clone 2F11.

Alternative Names
AAG6, MGC129900, MGC129901, PKC-alpha, PKCA, PRKACA

Size
100 µg

Purification
Protein A purified

Immunogen
PRKCA (NP_002728, 563 a.a. ~ 673 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG Mix Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (38.21 KDa).

 

Note: This item was formerly catalog no. ABB-7033 (ABB7033)

Applications

Western Blot	PRKCA Monoclonal Antibody, clone 2F11. Western Blot analysis of PRKCA expression in HeLa.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged PRKCA is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded human tonsil. [Antibody Concentration: 1 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to PRKCA on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.