Description
Mouse monoclonal antibody raised against a partial recombinant PLK4, clone 1C8.

Size
100 µg

Purification
Protein A purified

Immunogen
PLK4 (AAH36023, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml

*The end user is responsible for determining optimal working dilutions.

 

Note: This item was formerly catalog no. ABB-7112 (ABB7112) 

Applications

Western Blot	PLK4 Monoclonal Antibody, clone 1C8. Western Blot analysis of PLK4 expression in HeLa NE.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged PLK4 is approximately 0.03 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Not determined
Immunofluorescence	Not determined

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.