Description
Mouse monoclonal antibody raised against a partial recombinant PBK, clone 3A11.

Size
100 µg

Purification
Protein A purified

Immunogen
PBK (AAH15191, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml 

*The end user is responsible for determining optimal working dilutions.

 

Note: This item was formerly catalog no. ABB-7224 (ABB7224)

Applications

Western Blot	PBK Monoclonal Antibody, clone 3A11. Western Blot analysis of PBK expression in human skeletal muscle. PBK Monoclonal Antibody, clone 3A11. Western Blot analysis of PBK expression in A-431.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged PBK is approximately 1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis.  [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to PBK on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.