Description
Mouse monoclonal antibody raised against a full length recombinant NEK11, clone 4E1-1F4.

Size
100 µg

Purification
Protein A purified

Immunogen
NEK11 (AAH28587, 1 a.a. ~ 483 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (79.13 KDa).

 

Note: This item was formerly catalog no. ABB-7106 (ABB7106) 

Applications

Western Blot	NEK11 Monoclonal Antibody, clone 4E1-1F4. Western Blot analysis of NEK11 expression in HL-60. NEK11 Monoclonal Antibody, clone 4E1-1F4. Western Blot analysis of NEK11 expression in A-431.   Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 Monoclonal Antibody, clone 4E1-1F4. Lane 1: NEK11 transfected lysate (55.5 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Applicable
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded in human endometrium tissue. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.