Description
Mouse monoclonal antibody raised against a partial recombinant NEK10, clone 1C9.

Size
100 µg

Purification
Protein A purified

Immunogen
NEK10 (NP_689747, 211 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human, Mouse

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (36.01 KDa).

 

Note: This item was formerly catalog no. ABB-7105 (ABB7105)

Applications

Western Blot	NEK10 Monoclonal Antibody, clone 1C9. Western Blot analysis of NEK10 expression in HeLa. NEK10 Monoclonal Antibody, clone 1C9. Western Blot analysis of NEK10 expression in Raw 264.7. NEK10 Monoclonal Antibody, clone 1C9. Western Blot analysis of NEK10 expression in NIH/3T3. Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 Monoclonal Antibody, clone 1C9. Lane 1: NEK10 transfected lysate (53.1 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged NEK10 is approximately 0.03 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Not determined
Immunofluorescence	Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.