Description
Mouse monoclonal antibody raised against a partial recombinant MAPK13, clone 2D8.

Size
100 µg

Purification
Protein A purified

Immunogen
MAPK13 (AAH00433, 251 a.a. ~ 365 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2b Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (38.65 KDa).

 

Note: This item was formerly catalog no. ABB-7049 (ABB7049)

Applications

Western Blot	MAPK13 Monoclonal Antibody, clone 2D8. Western Blot analysis of MAPK13 expression in MCF-7. Western Blot analysis of MAPK13 expression in transfected 293T cell line by MAPK13 Monoclonal Antibody, clone 2D8. Lane 1: MAPK13 transfected lysate (42.1 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged MAPK13 is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to MAPK13 on formalin-fixed paraffin-embedded in human testis. [Antibody Concentration: 1 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to MAPK13 on MCF-7 cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.