c-IAP1 Monoclonal Antibody [1E1110]

Description
Rat monoclonal antibody to c-IAP1, Clone 1E1110

Formulation
Liquid. 0.2μm-filtered solution in PBS containing 0.05% sodium azide.

Immunogen
Synthetic peptide corresponding to aa 221-232 of mouse c-IAP1.

Specificity
Human, Mouse

Isotype
IgG2a

Purification
Serum

Storage
Store at +4°C for short term. Aliquot and store at -80°C for long term. Avoid multiple freeze/thaw cycles.

Alternative Names
HIAP2, BIRC-2, Baculoviral IAP repeat-containing protein-2, Cellular inhibitor of apoptosis-1, Human inhibitor of apoptosis protein-2, Inhibitor of apoptosis protein-2

Application
WB (1:1000-1:4000, ECL)

Fig. 1. Western blot analysis of c-IAP1 in mouse bone marrow-derived macrophages of the indicated strains lysed in DISC buffer (150mM sodium chloride, 2mM EDTA, 1% Triton X-100, 10% glycerol, 20mM Tris pH 7.5) using c-IAP1 Monoclonal Antibody [1E1110] followed by HRP-anti rat antibody and developed by ECL.

Fig. 2. Western blot analysis of human c-IAP1 usingc-IAP1 Monoclonal Antibody [1E1110] A: MDA-MB-231 cell lysate B: MDA-MB-231 +LBW 1μM/1h cell lysate (LBW causes degradation of c-IAP1; A. Gaither, et al., 2007)