Description
Mouse monoclonal antibody raised against a partial recombinant HIST2H2BE, clone 4G6.

Size
100 µg

Purification
Protein A purified

Immunogen
HIST2H2BE (NP_003519, 36 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2b Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml

*The end user is responsible for determining optimal working dilutions.

 

Note: This item was formerly catalog no. ABB-4033 (ABB4033)

Applications

Western Blot	Not determined
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged HIST2H2BE is approximately 3 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to HIST2H2BE on formalin-fixed paraffin-embedded human placenta. [Antibody Concentration: 5 µg/ml]
Immunofluorescence	Not determined

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.