Description
Mouse monoclonal antibody raised against a full length recombinant HDAC8, clone 2F4.

Size
100 µg

Purification
Protein A purified

Purification
Protein A purified

Immunogen
HDAC8 (AAH50433, 1 a.a. ~ 378 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2b Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (67.58 KDa).

 

Note: This item was formerly catalog no. ABB-4008 (ABB4008)

Applications

Western Blot	HDAC8 Monoclonal Antibody [2F4]. Western Blot analysis of HDAC8 expression in Hela NE. Western Blot analysis of HDAC8 expression in transfected 293T cell line by HDAC8 Monoclonal Antibody [2F4]. Lane 1: HDAC8 transfected lysate (41.8 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged HDAC8 is approximately 0.3 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Not determined
Immunofluorescence	Immunofluorescence of monoclonal antibody to HDAC8 on HeLa cell.  [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.