Description
Mouse monoclonal antibody raised against a full length recombinant H3F3B, clone 2D7-H1.

Size
100 µg

Purification
Protein A purified
 

Immunogen
H3F3B (AAH17558, 1 a.a. ~ 137 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (41.07 KDa).

 

Note: This item was formerly catalog no. ABB-4035 (ABB4035)

Applications

Western Blot	Western Blot analysis of H3F3B expression in transfected 293T cell line by H3F3B Monoclonal Antibody [2D7-H1]. Lane 1: H3F3B transfected lysate (15 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Applicable
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver.
Immunofluorescence	Immunofluorescence of monoclonal antibody to H3F3B on HeLa cell.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.