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EpiSonic 2000 Sonication System

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For multi-sample shearing of DNA and chromatin in next-generation sequencing applications

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Demonstration of consistent fragments achieved with a single run of 12-sample multiplex shearing with the EpiSonic™ 2000.
Setup of the EpiSonic™ 2000 Sonication System.
Reproducibility at 150 bp shearing
Reproducibility at 300 bp shearing
Reproducibility at 350 bp shearing
Reproducibility at 2 kb shearing
Reproducibility at 6 kb shearing
Reproducibility at 20 kb shearing
Purpose: Sample Fragmentation
Catalog No.SizePriceQty
EQC-20001 unit $16,428.00 
 
Availability: Ships Within 30 Days 
Product Overview

The EpiSonic™ 2000 Sonication System is the latest sonication-based instrument with integrated cooling for DNA shearing and chromatin shearing applications. This sonicator is designed for simultaneous, optimized processing of 1 to 12 samples at small microliter-level volumes (optimal at 15 µl of volume), making it an ideal solution for next-generation sequencing due to its high shearing consistency and uniformity.

Features At-A-Glance
The EpiSonic™ 2000 incorporates Digitally Adaptive Sonocavitation™ (DAS) technology to efficiently process DNA and chromatin samples, with the following key features:

  • Simple input and controls
  • Simultaneous sonication of up to 12 samples
  • 10 minutes to shear 12 samples to 300 bp (50 sec/sample)
  • Reproducible and consistent fragments
  • Closed vessel processing
  • Easy installation and maintenance
  • Continuous cooling system
  • 20-month warranty

Key Points
Notable points of the EpiSonic™ 2000 include the following:

  • Generates fragments compatible with IlluminaIon Torrent454, and Pacific Biosciences platforms.
  • Generates tight and highly reproducible fragment distributions.
  • Capable of achieving uniform fragment sizes between 150 bps and 20 kbs.
  • Meets "the scientist's requirements" for a low-cost, flexible, higher throughput and easy-to-use design.
  • Eliminates operator-induced variations, improves recovery yield, increases efficiency, and provides results that can be standardized.

High throughput sonication

Fig. 2. Demonstration of consistent fragments achieved with a single run of 12-sample multiplex shearing with the EpiSonic™ 2000.


DAS™ technology, incorporated into the EpiSonic™ 2000, provides the following advantages:

  • Controlled energy delivery
  • Isothermal environment
  • Non-contact, closed vessel processing
  • Enhanced reproducibility
  • Uniform multiplex sample shearing
  • User selectable fragment lengths
  • High sample recovery with no heat damage
  • No cross-contamination, no aerosols, no clean up
  • Micro-scale sample volumes
  • Real-time process monitoring
High throughput sonication

Fig. 1. Setup of the EpiSonic™ 2000 Sonication System. 


Specifications

Model Number 2000
Input Voltage 100-120 VAC or 220-240 VAC @ 50/60 Hz
Power Rating 750 Watts
Programmable Timer Up to 10 hours
Operating Frequency 20 kHz
Timer Control Digital
Throughput Capability Up to 12
Chiller Dimensions  7.5"L x 5"W x 7"H (19 cm x 12.7 cm x 17.6 cm)
Generator Dimensions 15.25"L x 8"W x 8.5"H (38.7 cm x 20.3 cm x 21.6 cm)
Sound Enclosure Dimensions 10"L x 10"W x 16.5"H (25.5 cm x 25.5 cm x 42 cm)
Operating Environment Temperature: 39°-95°F (4°-35°C)
Relative Humidity 20-90% (Non Condensing)
For indoor use only
Origin of Manufacture USA
Warranty Period 20 months

Reproducibility at 150 bp shearing Reproducibility at 300 bp shearing Reproducibility at 350 bp shearing

Fig 2

Fig 3

Fig 4

 

Reproducibility at 2 kb shearing

 

Reproducibility at 6 kb shearing

 

Reproducibility at 20 kb shearing

Fig 5 Fig 6 Fig 5
Product Components

Sonication Generator (100-120V) (Part #EQC-2000-PSG); or
Sonication Generator (220-240V) (Part #EQC-2000-PSG2) 
Sample Processing Horn (Part  #EQC-2000-PPH)
Soundproof Enclosure (Part #EQC-2000-PSE)
Ultrasonic Converter (Part #EQC-2000-PUC)
Recirculating Chiller (Part #EQC-2000-PRC)
Power Cord (US) (Part #EQC-2000-PPC); or
Power Cord (UK) (Part #EQC-2000-PPCUK); or
Power Cord (Euro) (Part #EQC-2000-PPCEU)
Flow Tubing with Fittings Set of 2 (Part #EQC-2000-PFT)
Water Release Connector (Part #EQC-2000-PWR)
Line Filter Release Connector (Part #EQC-2000-PFR)
Line Filter (Part #EQC-2000-PLF)
Filter Cartridges (4/pack) (Part #EQC-2000-PFC)
Converter Cable (Part  #EQC-2000-PCC)
Assembly Wrench Set of 2 (Part #EQC-2000-PSW)
0.2 ml Tube Rack (Part #EQC-2000-PTR)
Starter Vessel Pack 

Frequently Asked Q's

What is ultrasonics?
Ultrasonics, or ultrasound, literally means beyond sound -- sound above the human audible spectrum. Standard ultrasonic processors or sonicators operate at a nominal frequency of 20 kilohertz (kHz) or 20,000 cycles per second (cps). The automatic tuning feature of the EpiSonic 2000 actually moves the frequency within a small range during operation to optimize performance. The ultrasonic energy can then be converted by a pziezoelectric converter/transducer into acoustic energy to cause liquid cavitation through mechanical vibrations.

Why does the EpiSonic operate at 20kHz?
At lower range frequencies (20kHz to 100kHz), large cavitation bubbles are produced -- the collapse of which causes extreme hydraulic shearing forces and effects. Digitally Adaptive Sonocavitation™ (DAS), a 20kHz principle, allows for the size of the cavitation bubbles and the corresponding cavitation energy produced at low acoustic frequencies to be digitally controlled by dynamic amplitude adjustments of 1% to 100%. Therefore, DAS™ is more suitable for generating controllable shearing forces to break up cells and macromolecules such as DNA and chromatin in a non-contact, precise, and reproducible manner.  

What are the differences between the lower frequency Digitally Adaptive Sonocavitation (DAS) and higher frequency mechanisms for acoustic-based sonication?
DAS™ operates at 20kHz to cater to users who desire flexibility in being able to powerfully shear samples at fast speeds as well as to precisely shear samples at slower speeds. In frequencies of levels higher than 20kHz (e.g., medium frequency range of 100kHz to 1000kHz such as Adaptive Focused Acoustics™ or AFA), there is less time for the cavitation bubbles to grow. This unfortunately results in smaller bubbles and a corresponding reduction in cavitation energy with increased wait times to effectively shear samples. 100kHz to 1000 kHz frequencies are instead better suited for submicron particle removal and often used for breaking up the structure of organic compounds such as chlorophenol, TBT, and MTBE. Higher frequency sonication does not generally hold any advantages over standard 20kHz frequency sonication for shearing DNA or chromatin. The DAS™ technology of the EpiSonic 2000 provides maximum versatility by combining the powerful shearing forces of 20kHz frequency with digitally precise amplitude controls to finely adjust the intensity of such forces.

Can the EpiSonic be used with any other applications?
Although the EpiSonic 2000 was specifically designed and supported for DNA shearing, chromatin shearing, and cell disruption/lysis, it is capable of disaggregation, homogenization, cleaning, mixing, degassing, dispersion, cleavage, catalyzing reactions, hydrolyzation, extraction, dissolution, emulsification, comminution, synthesis, microencapsulation.

What kind of volume sizes can be used with the EpiSonic per sample?
0.2 ug of DNA in 15 µl of volume is recommend per processing tube.

 

Adaptive Focused Acoustics is a trademark of Covaris, Inc.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Diallo K et. al. (May 2017). Hierarchical genomic analysis of carried and invasive serogroup A Neisseria meningitidis during the 2011 epidemic in Chad. BMC Genomics. 18(1):398.

Upadhyaya B et. al. (April 2017). Prenatal Exposure to a Maternal High-Fat Diet Affects Histone Modification of Cardiometabolic Genes in Newborn Rats. Nutrients. 9(4)

Johnson EC et. al. (October 2016). A small molecule targeting protein translation does not rescue spatial learning and memory deficits in the hAPP-J20 mouse model of Alzheimer's disease. PeerJ. 4:e2565.

Schmidt K et. al. (September 2016). Identification of bacterial pathogens and antimicrobial resistance directly from clinical urines by nanopore-based metagenomic sequencing. J Antimicrob Chemother.

Weyand NJ et. al. (September 2016). Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse. Int J Syst Evol Microbiol. 66(9):3585-3593.

Mukherjee A et. al. (November 2015). HMGB1 interacts with XPA to facilitate the processing of DNA interstrand crosslinks in human cells. Nucleic Acids Res.

Morris M et. al. (October 2015). Network dysfunction in α-synuclein transgenic mice and human Lewy body dementia Ann Clin Transl Neurol..

Xu X et. al. (June 2015). Hypoxia-induced miR-181b enhances angiogenesis of retinoblastoma cells by targeting PDCD10 and GATA6. Oncol Rep. 33(6):2789-96.

Deasy AM et. al. (May 2015). Nasal Inoculation of the Commensal Neisseria lactamica Inhibits Carriage of Neisseria meningitidis by Young Adults: A Controlled Human Infection Study. Clin Infect Dis. 60(10):1512-20.

Gheyara AL et. al. (July 2014). Tau reduction prevents disease in a mouse model of Dravet syndrome. Ann Neurol.

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