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EpiQuik In Situ H3K9 Acetylation Assay Kit

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Schematic procedure for using the EpiQuik In Situ H3K9 Acetylation Assay Kit.
Log-phase MCF-7 cells were treated with trichostatin A (TSA, 0.5
Input Type: Cells
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-4004-09696 assays $421.00 
P-4004-1922x96 assays $658.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ In Situ H3-K9 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure in situ acetylation of histone H3-K9 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ In Situ H3-K9 Acetylation Assay Kit is suitable for specifically measuring in situ histone H3-K9 acetylation using cultured adherent cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Measurement of in situ histone H3-K9 acetylation, with out the need to prepare cell lysates.
  • Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits H3-K9 acetylation.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ Histone H3-K9 Acetylation Assay Kit uses whole cell-based detection of acetylated H3-K9. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The acetylated H3-K9 is then detected by a high affinity acetyl H3-K9 antibody. The ratio or amount of acetyl H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Product Components

GH1 (10X Wash Buffer)
GH2 (Permeabilizing Buffer)
GH3 (Blocking Buffer)
GH4 (Antibody Buffer)
GH5 (Capture Antibody, 1 mg/ml)*
GH6 (Detection Antibody, 400 µg/ml)*
GH7 (Developing Solution)
GH8 (Stop Solution)
30% H2O2 Solution
Acetylated H3-K9 Control (20 µg/ml)
8-Well Control Strips
Microplates
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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