The EpiQuik™ In Situ H3-K9 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure in situ acetylation of histone H3-K9 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ In Situ H3-K9 Acetylation Assay Kit is suitable for specifically measuring in situ histone H3-K9 acetylation using cultured adherent cells.

WHY CHOOSE THE EPIQUIK™ IN SITU H3-K9 ACETYLATION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Measurement of in situ histone H3-K9 acetylation, with out the need to prepare cell lysates.
• Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits H3-K9 acetylation.
• Simple, reliable, and consistent assay conditions.

EpiQuik™ In Situ Histone H3-K9 Acetylation Assay Kit uses whole cell-based detection of acetylated H3-K9. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The acetylated H3-K9 is then detected by a high affinity acetyl H3-K9 antibody. The ratio or amount of acetyl H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:
	Log-phase MCF-7 cells were treated with trichostatin A (TSA, 0.5 µM for 24 h). The level of acetyl histone H3-K9 was measured with this kit.

GH1 (10X Wash Buffer)
GH2 (Permeabilizing Buffer)
GH3 (Blocking Buffer)
GH4 (Antibody Buffer)
GH5 (Capture Antibody, 1 mg/ml)*
GH6 (Detection Antibody, 400 µg/ml)*
GH7 (Developing Solution)
GH8 (Stop Solution)
30% H2O2 Solution
Acetylated H3-K9 Control (20 µg/ml)
8-Well Control Strips
Microplates
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]