The EpiQuik™ In Situ Histone H3-K4 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K4 methylation in situ using cultured adherent cells.

WHY CHOOSE THE EPIQUIK™ IN SITU HISTONE H3-K4 METHYLATION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Measurement of in situ histone H3-K4 methylation without the need to prepare cell lysates.
• Microplate format makes the assay suitable for high throughput analysis of agents that increase or inhibit H3-K4 methylation.
• Simple, reliable, and consistent assay conditions.

EpiQuik™ In Situ Histone H3-K4 Methylation Assay Kit is a whole cell-based detection of methylated H3-K4. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3-K4 is then detected by an anti-methyl H3-K4 antibody. The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

GA1 (10X Wash Buffer)
GA2 (Permeabilizing Buffer)
GA3 (Blocking Buffer)
GA4 (Antibody Buffer)
GA5 (Capture Antibody, 100 µg/ml)*
GA6 (Detection Antibody, 200 µg/ml)*
GA7 (Developing Solution)
GA8 (Stop Solution)
30% H2O2 Solution
Methylated H3-K4 Control (20 µg/ml)
8-Well Control Strips
Microplates
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]