Product Overview
The EpiQuik™ Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global tri-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYL HISTONE H3-K9 QUANTIFICATION KIT (COLORIMETRIC)?
- Quick and efficient procedure, which can be finished within 2.5 hours.
- Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
- Specifically captures tri-methylated H3-K9 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
- The control is conveniently included for the quantification of the amount of tri-methylated H3-K9.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
Product Details
The EpiQuik™ Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is designed for measuring global histone H3-K9 tri-methylation. In an assay with this kit, the tri-methylated histone H3 at lysine 9 is captured to the strip wells coated with an anti-tri-methyl H3-K9 antibody. The captured tri-methylated histone H3-K9 can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of tri-methylated H3-K9 is proportional to the intensity of absorbance. The absolute amount of tri-methylated H3-K9 can be quantitated by comparing to the standard control.
| SCHEMATIC PROCEDURE: |
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 Histone extracts were prepared from MDA-MB-231 cells using the EpiQuik Total Histone Extraction Kit and the amount of trimethyl-H3-K9 was measured using the EpiQuik Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric). |
Product Components
C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detection Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide
* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
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