The EpiQuik™ Global Pan-Methyl Histone H3-K36 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global tri-methylation of histone H3-K36 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYL HISTONE H3-K36 QUANTIFICATION KIT (FLUOROMETRIC)?

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative fluorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Specifically capturies tri-methylated H3-K36 with the detection limit as low as 0.4 ng/well and detection range from 5 ng-2 µg/well of histone extracts.
• The control is conveniently included for the quantification of the amount of tri-methylated H3-K36.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global Tri-Methyl Histone H3-K36 Quantification Kit (Fluorometric) is designed for measuring global histone H3-K36 tri-methylation. In an assay with this kit, the tri-methylated histone H3 at lysine 36 is captured to the strip wells coated with an anti-tri-methyl H3-K36 antibody. The captured tri-methylated histone H3-K36 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of tri-methylated H3-K36 is proportional to the intensity of fluorescence. The absolute amount of tri-methylated H3-K36 can be quantified by comparing to the standard control.

SCHEMATIC PROCEDURE:

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.