The EpiQuik Global Pan-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is a complete set of essential components which enables the experimenter to measure global mono-, di- and tri-methylation of histone H4-K20. H4-K20 mono-methylation is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, as well as functioning in gene silencing. H4-K20 di-methylation has been described as a repressive chromatin domain and is involved in DNA damage response. H4-K20 tri-methylation acts as a passive feature or structure determinant for chromatin degradation and release and is an epigenetic marker of early apoptosis. The global H4-K20 methylation can be changed by inhibiton or activation of HMTs, thus quantitative detection of global methyl histone H4-K20 would provide useful information for better understanding epigenetic regulation of gene activation/repression as well as for developing HMT-targeted drugs.

WHY CHOOSE THE EPIQUIK GLOBAL PAN-METHYL HISTONE H4-K20 QUANTIFICATION KIT (FLUOROMETRIC)?

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative fluorometric assay with no need for radioactivity, electrophoresis, or chromatography.
• Simultaneously quantify mono-, di-, and tri-methylated H4-K20 with the detection limit as low as 1 ng/well, and detection range from 10 ng-2 µg/well of histone extracts.
• The control is conveniently included for quantification of pan-methylated H4-K20.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik Global Pan-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is designed for simultaneously measuring mono-, di-, and tri-methylation of histone H4-K20. In an assay with this kit, the mono-, di-, and tri-methylated histone H4 at lysine 20 is captured to the strip wells coated with anti-mono-, di-, and tri-methyl H4-K20 antibody. The captured methylated histone H4-K20 can then be detected with a detection antibody followed by a fluorescent development reagent. The ratio of mono-, di-, and tri-methylated H4-K20 is proportional to the intensity of fluorescence. The absolute amount of mono-, di-, and tri-methylated H4-K20 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:

F1 (10X wash buffer)
F2 (antibody buffer)
F3 (detecting antibody, 1 mg/ml)*
F4 (fluoro developer)*
F5 (fluoro enhancer)*
F6 (fluoro dilution)
Standard control (100 µg/ml)*
Signal report solution*
Signal enhancer*
8 well sample strips (with frame)
8 well standard control strips
User guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]