The EpiQuik™ Global Histone H3-K27 Tri-Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K27 TRI-METHYLATION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global H3-K27 Tri-Methylation Assay Kit is designed for measuring global histone H3-K27 tri-methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The tri-methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

GT1 (10X Lysis Buffer)
GT2 (Extraction Buffer)
GT3 (10X Wash Buffer)
GT4 (Histone Buffer)
GT5 (Blocking Buffer)
GT6 (Antibody Buffer)
GT7 (Capture Antibody, 100 µg/ml)*
GT8 (Detection Antibody, 400 µg/ml)*
GT9 (Developing Solution)
GT10 (Stop Solution)
Tri-Methyl H3-K27 Control (60 µg/ml)*
8-Well Assay Strips (with frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Deligezer, U. et. al. (October 2010). Post-treatment circulating plasma BMP6 mRNA and H3K27 methylation levels discriminate metastatic prostate cancer from localized disease. Clin Chim Acta. 411(19-20): 1452-6. PubMed Abstract