We recommend using a newer version of this product: EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit

The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is a convenient package of tools that allows the experimenter to measure DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition. The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is suitable for measuring DNA demethylase activity/inhibition using purified enzyme or protein extracts from the cultured cells and tissues.

WHY CHOOSE THE EPIQUIK™ DNA DEMETHYLASE ACTIVITY/INHIBITION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is designed for measuring total DNA demethylase activity/inhibition. In an assay with this kit, the unique methylated DNA substrate is stably captured on the strip wells. Active DNA demethylases bind to and demethylate DNA substrate. The methylated DNA can be recognized with a high affinity 5-methycytosine antibody. The ratio or amount of methylated DNA, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction. SCHEMATIC PROCEDURE:

Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total DNA demethylase activity was measured.

DD1 (10X Wash Buffer)
DD2 (Demethylase Assay Buffer)
DD3 (Demethylase Substrate)*
DD4 (Demethylation Standard, 10 µg/ml)*
DD5 (Capture Antibody, 1000 µg/ml)*
DD6 (Detecting Antibody, 200 µg/ml)*
DD7 (Developing Solution)
DD8 (Stop Solution)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Horrillo, A. et. al. (April 2013). Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment. Cell Death Dis. 4:e570. PubMed Abstract

Kahaleh, B. et. al. (2012). Decrease activity of DNA demethylase in SSC fibroblast and microvascular endothelial cells: a possible mechanism for persistent SSC phenotype. Rhemuatology. Abstract