Product Overview
The
EpiQuik™ In Situ Histone H3 Phosphorylation (Ser10) Assay Kit is a convenient set of tools that allows the experimenter to measure
in situ phospho histone H3 (ser10). The kit is ready-to-use and provides all the essential components needed for specifically measuring histone H3 phosphorylation (ser10)
in situ using cultured adherent cells.
WHY CHOOSE THE EPIQUIK™ IN SITU HISTONE PHOSPHORYLATION (SER10) ASSAY KIT?
- Quick and efficient, which can be finished within 3 hours.
- Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
- Measurement of in situ histone H3 phosphorylation at ser10 and no need to prepare cell lysates.
- Microplate format makes the assay suitable for high throughput analysis of reagents that increases or inhibits histone H3 phosphorylation at ser10 .
- Simple, reliable, and consistent assay conditions.
Principle & Procedure
EpiQuik™
In Situ Histone H3 phosphorylation (Ser10) Assay Kit is a whole cell-based detection of histone H3 phosphorylation (ser10) . In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The histone H3 phosphorylation (ser10) is then detected by anti-phospho histone H3 (ser10) antibody. The ratio or amount of phospho histone H3 (ser10) can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Product Components
PA1 (10x wash buffer)
PA2 (Permeabilizing buffer)
PA3 (Blocking buffer)
PA4 (Antibody buffer)
PA5 (capture antibody, 1000 µg/ml)*
PA6 (detection antibody, 200 µg/ml)*
PA7 (developing solution)
PA8 (stop solution)
30% H2O2 solution
Phospho H3
ser10 control (20 µg/ml)
8-well control strips
Microplates
User guide
* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
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