Description
Mouse monoclonal antibody raised against a full length recombinant EIF2C2, clone 2E12-1C9.

Alternative Names
AGO2,MGC3183,Q10

Size
100 µg

Purification
Protein A purified

Immunogen
EIF2C2 (AAH07633, 1 a.a. ~ 377 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (68.4 KDa).

 

Note: This item was formerly catalog no. ABB-5001 (ABB5001)

Applications

Western Blot	Applicable
Immunoprecipitation	Not determined
ELISA	Applicable
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded in human stomach. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Cheloufi, S. et. al. (June 2010). A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature. 465(7298): 584-9. PubMed Abstract

Flemr, M. et. al. (May 2010). P-body loss is concomitant with formation of a messenger RNA storage domain in mouse oocytes. Biol Reprod. 82(5): 1008-17. PubMed Abstract

Lin, J. C. et. al. (December 2009). RNA-binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 during muscle cell differentiation. J Biol Chem. 284(50): 34658-65. PubMed Abstract

Tan, L. P. et. al. (November 2009).  A high throughput experimental approach to identify miRNA targets in human cells. Nucleic Acids Res. 37(20): e137. PubMed Abstract

Landry, P. et. al. (September 2009). Existence of a microRNA pathway in anucleate platelets. Nat Struct Mol Biol. 16(9): 961-6. PubMed Abstract

Zeitelhofer, M. et. al. (July 2008). Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons. J Neurosci. 28(30): 7555-62. PubMed Abstract

Alisch, R. S. et. al. (December 2007). Argonaute2 is essential for mammalian gastrulation and proper mesoderm formation. PLoS Genet. 3(12): e227. PubMed Abstract

Vickers, T. A. et. al. (September 2007). Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells. Nucleic Acids Res. 35(19): 6598-610. PubMed Abstract

Krol, J. et. al. (February 2007). Ribonuclease dicer cleaves triplet repeat hairpins into shorter repeats that silence specific targets. Mol Cell. 25(4): 575-86. PubMed Abstract