BackgroundThis gene is specifically involved in the methylation of aspartic acid transfer RNA at the cytosine position 38. Although it is considered part of the family of DNA cytosine methyltransferases, DNMT2 does not methylate CpG in genomic DNA because it lacks the large N-terminal regulatory domain found in other eukaryotic methyltransferases.
DescriptionMouse monoclonal antibody raised against a full length recombinant DNMT2 (also known as TRDMT1), clone 1E12.
Size100 µg
PurificationProtein A purified
ImmunogenDNMT2 (AAH47733, 1 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Lambda
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesdDNMT antibody, DmMT 2 antibody, DNA (cytosine 5) methyltransferase like protein 2 antibody, DNA 5 cytosine methyltransferase antibody, DNA methyltransferase 2 antibody, DNA methyltransferase homolog HsaIIP antibody, DNA MTase homolog HsaIIP antibody, Dnmt 2 antibody, MHsaIIP antibody, nmt 2 antibody, OTTHUMP00000045198 antibody, PuMet antibody, RNMT 1 antibody, TRDMT 1 antibody, TRDMT_HUMAN antibody, tRNA (cytosine 5 ) methyltransferase antibody, tRNA (cytosine(38)-C(5))-methyltransferase antibody, tRNA aspartic acid methyltransferase 1 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 μg/mlELISA: 0.25-1 μg/mlImmunofluorescence: 5 μg/ml
*The end user is responsible for determining optimal working dilutions.
Quality Control
Western Blot detection against Immunogen (69.12 KDa).
Note: This item was formerly catalog no. ABB-1002 (ABB1002)
Applications
Western Blot
DNMT2 Monoclonal Antibody, clone 1E12 Western Blot analysis of DNMT2 expression in HeLa NE.Western Blot analysis of DNMT2 expression in transfected 293T cell line by DNMT2 monoclonal antibody, clone 1E12. Lane 1: DNMT2 transfected lysate (45 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation
ELISA
Immunohistochemistry (IHC)