Description
Mouse monoclonal antibody raised against a partial recombinant CDKN1A, clone 2F1.

Alternative Names
CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1

Size
100 µg

Purification
Protein A purified

Immunogen
CDKN1A (NP_000380, 65 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (37.11 KDa).

 Note: This product was formerly catalog no. ABB-2087 (ABB2087)

Applications

Western Blot	CDKN1A Monoclonal Antibody, clone 2F1. Western Blot analysis of CDKN1A expression in human liver.
Immunoprecipitation	Not Determined
ELISA	Detection limit for recombinant GST tagged CDKN1A is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Not Determined
Immunofluorescence	Immunofluorescence of monoclonal antibody to CDKN1A on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.