Description
Mouse monoclonal antibody raised against a partial recombinant CDC25C, clone 3B11.

Size
100 µg

Purification
Protein A purified

Immunogen
CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (38.1 KDa).

 

Note: This item was formerly catalog no. ABB-7266 (ABB7266)

Applications

Western Blot	CDC25C Monoclonal Antibody, clone 3B11. Western Blot analysis of CDC25C expression in HeLa. Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C Monoclonal Antibody, clone 3B11. Lane 1: CDC25C transfected lysate (53.312 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged CDC25C is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [Antibody Concentration: 6 µg/ml]
Immunofluorescence	Not determined

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.