Caspase-1 Monoclonal Antibody [4G8]

Description
Rat monoclonal antibody to Caspase-1, Clone 4G8

Formulation
Liquid. Protein G Sepharose™ purified antibody in PBS containing 0.02% sodium azide.

Immunogen
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T206ALEMVKEVKEFAAC220) of mouse caspase-1.

Specificity
Mouse

Isotype
IgG1

Purification
Protein G-Sepharose purified.

Storage
Store at +4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.

Alternative Names
IL-1B converting enzyme, Interleukin-1B converting enzyme, ICE

Application
ELISA, FC, WB

Fig. 1. Caspase-1 Monoclonal Antibody [1H11] and Caspase-1 Monoclonal Antibody [4G8] detect overexpressed caspase-1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse caspase-1 as a band of ~45 kDa.

Fig. 2. Monoclonal antibody to caspase-1 [1H11] and Caspase-1 Monoclonal Antibody [4G8] detect endogenous pro-caspase-1 in spleen from WT mice, but not from spleen of caspase-1 deficient mice. HSP-70: loading control.