Caspase-8 Monoclonal Antibody [3B10]

Background
Caspase-8 and the sequential activation of caspases such as caspase-1, 3, 6, and 7, is important to the processes of cell apoptosis, or programmed cell death. The caspase-8 protein is thought to interact with Fas-interaction protein FADD and may also play a role in neurodegenerative diseases such as Huntington disease. 

Description
Rat monoclonal antibody to Caspase-8, Clone 1G12

Formulation
Liquid. In PBS containing 0.02% sodium azide.

Immunogen
Recombinant mouse caspase-8 p18 subunit

Specificity
Mouse

Isotype
IgG1

Purification
Protein G- affinity purified.

Storage
Store at +4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.

Alternative Names
FLICE

Application
ELISA, FC, ICC, WB

Fig. 1. Caspase-8 Monoclonal Antibody [3B10] and [1G12] both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.