Bisulfite and sodium bisulfite based techniques, often known as bisulfite conversion, are common approaches for preparing DNA for gene-specific DNA methylation analysis. Treatment of DNA with bisulfite converts cytosine to uracil while leaving 5-methylcytosine intact, allowing for single-nucleotide resolution information about the methylated areas of DNA. Popular methods for analyzing DNA methylation on a gene-specific basis, after bisulfite treatment, include bisulfite sequencing, methylation specific PCR, and methylation-based microarrays.

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