BimS/EL/L Monoclonal Antibody [3C5]

Description
Rat monoclonal antibody to BimS/EL/L, Clone 3C5

Formulation
Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.

Immunogen
Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.

Specificity
Human, Mouse, Rat, Monkey, Dog

Isotype
IgG2a

Purification
Protein G- affinity purified.

Storage
Store at +4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.

Alternative Names
BCL2L11

Application
FC, ICC, IP (2 µg/ml), WB (1-5 µg/ml)

Fig. 1. Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat BimS/EL/L Monoclonal Antibody [3C5] the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control).

Fig. 2. Western blotting with BimS/EL/L Monoclonal Antibody [3C5] recognizes all known isoforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with BimS/EL/L Monoclonal Antibody [3C5].

Fig. 3. BimS/EL/L Monoclonal Antibody [3C5] detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice.