BimS/EL/L Monoclonal Antibody [10B12]

Description
Rat monoclonal antibody to Bim/S/E/L, Clone 10B12

Formulation
Liquid. PBS containing 25% glycerol and 0.02% sodium azide.

Immunogen
Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.

Specificity
Human, Mouse, Rat, Monkey, Dog

Isotype
IgG2a

Purification
Protein G-affinity purified.

Storage
Store at +4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.

Alternative Names
BCL2L11

Application
ELISA, FC, ICC, IHC, IP (2 µg/ml), WB (1-5 µg/ml)

Fig. 1. Western blotting with BimS/EL/L Monoclonal Antibody [10B12] recognizes all known isforms of Bim.

Fig. 2. BimS/EL/L Monoclonal Antibody [10B12] detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) by Western blotting with BimS/EL/L Monoclonal Antibody [10B12] and detection by ECL.