BackgroundTranscription regulator BCL11A (B-cell CLL/lymphoma 11A) plays a critical role in lymphopoiesis and affects the progression of hematopoietic malignancies. BCL11A regulates hemoglobin gene expression during fetal development and is involved in silencing of γ-globin expression in adults. It also interacts with the Mi-2/NuRD chromatin remodeling complexes and erythroid transcription factors GATA1 and FOG1. Excess expression of this gene is developmentally restricted to adult erythroid cells. Multiple transcript variants exist for this gene.
DescriptionMouse monoclonal antibody raised against a partial recombinant BCL11A, clone 3D9.
Size100 µg
PurificationProtein A purified
ImmunogenBCL11A (NP_060484, 1 a.a. ~ 89 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG2a Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative Names2810047E18Rik antibody, B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody, B cell lymphoma/leukaemia 11A antibody, BCL11A L antibody, BCL11A S antibody, BCL11A XL antibody, C2H2 type zinc finger protein antibody, COUP TF interacting protein 1 antibody, Ctip 1 antibody, D930021L15Rik antibody, Ecotropic viral integration site 9 antibody, Evi 9 antibody, FLJ10173 antibody, FLJ34997 antibody, HBFQTL5 antibody, KIAA1809 antibody, mKIAA1809 antibody, OTTHUMP00000159788 antibody, OTTHUMP00000159789 antibody, OTTHUMP00000201250 antibody, OTTHUMP00000202084 antibody, Zinc finger protein 856 antibody, ZNF856 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunohistochemistry (IHC): 2.5 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (35.79 KDa).
Note: This item was formerly catalog no. ABB-4024 (ABB4024)
Applications
Western Blot
Western Blot analysis of BCL11A expression in transfected 293T cell line by BCL11A Monoclonal Antibody [3D9].Lane 1: BCL11A transfected lysate (26.865 KDa).Lane 2: Non-transfected lysate.