We recommend an alternative version of this product: ACTB Monoclonal Antibody
Background Beta actin, or cytoplasmic actin, is a 43kDa highly conserved protein that is ubiquitously expressed in all eukaryotic cells. Actins, along with microtubules, comprise a major component of the cytoskeleton. B-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of Beta-actin is generally used as an internal control for experimental error.
DescriptionMouse monoclonal antibody to B-actin, clone A2-F6
SpecificityHuman, Mouse, Rat
IsotypeIgG1
Uniprot IDP60709
PurificationAffinity Purified
ImmunogenSynthetic peptide (KLH-coupled)
FormulationLiguid. 1*TBS (pH7.4), 0.5% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at 4°C after thawing. Aliquot store at -20°C (regular) or -80°C (long-term). Avoid repeated freeze / thaw cycles.
Alternative NamesCytoplasmic Actin , ACTB, Actin, Actin, Beta, Cytoplasmic 1, B actin, Beta actin, F actin, β actin, Anti B-actin
ApplicationWB: 1:10,000ICC: 1:100-1:200IHC: 1:100-1:200FC: 1:100-1:200
JHB0002