Mouse monoclonal antibody raised against a synthetic peptide corresponding to the phosphorylated histone H3 at Ser10, ChIP-grade
Protein A purified
Detects histone H3 only when phosphorylated at Ser10 in mouse, rat, and human. Broad species cross-reactivity predicted based on sequence homology
PBS (pH 7.5), 150 mM NaCl, 30% glycerol
-20°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance
WB: 1:1000-1:2000, IF: 1:100-1:500, IHC: 1:100-1:500, ELISA: 1:1000-1:2000, IP: 2 μg/106 cells
Fig. 1. WB analysis of Phospho-Histone H3 (Ser10) Monoclonal Antibody with HeLa cell lysates (A-4049).