DNA Methylation at cytosine residues has a role in regulation of gene expression, genomic imprinting and is vital for mammalian growth. Hypermethylation of CpGisland in tumor repressor genes or hpyomethylaiton of mass genomic DNA has been linked to cancer development. Three families of DNMT genes are known which include DNMT1, DNMT2 and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic death. DNMT1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites indicating a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway which is abnormal in nearly all cancer cells.
Mouse monoclonal antibody to Dnmt1, clone 60B1220.1, ChIP-grade
Protein A purified
0.05% Sodium Azide, 0.2% Gelatin, PBS
4ºC, stable for 1 year from the date of shipment. For long-term storage, aliquot and store at -20ºC. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance
ADCADN antibody, AIM antibody, CXXC finger protein 9 antibody, CXXC-type zinc finger protein 9 antibody, CXXC9 antibody, DNA (cytosine-5)-methyltransferase 1 antibody, DNA methyltransferase 1 antibody, DNA methyltransferase HsaI antibody, DNA methyltransferase M.HsaI. antibody, DNA MTase antibody, DNA MTase HsaI antibody, DNMT 1 antibody, DNMT antibody, Dnmt1 antibody, DNMT1_HUMAN antibody, Dnmt1o antibody, FLJ16293 antibody, HSN1E antibody, M.HsaI antibody, MCMT antibody, Met1 antibody, MGC104992 antibody, mMmul antibody, MommeD2 antibody
WB: 1:250; IF: 1:100-1: 500; ELISA: 1:1000-1:2000, IP: 2 μg/106 cells
Fig. 1. WB analysis of DNMT1 Monoclonal Antibody (A-1001, 1:1000) with use of Hela cell extracts.