BackgroundActin is found in essentially all eukaryotic cells. It is one of the main proteins of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape, as well as the motility, of other cells. Actin is an effective loading control used in Western blotting experiments. In higher eukaryotes, there are six isoforms of actin: Three alpha-actins (α-skeletal, α-cardiac, α-smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and γ-non-muscle).
DescriptionRabbit polyclonal antibody to Actin
FormulationLiquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
ImmunogenBovine skeletal muscle Actin.
SpecificityHuman, Mouse, Rat, Bovine, Dog, Chicken, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus
PurificationProtein A-affinity purified.
StorageStore at -20°C. Avoid multiple freeze/thaw cycles.
Alternative NamesACTA, ACTSA
ApplicationWB (1:1000, ECL)
Fig. 1. Western Blot Analysis of Actin: Lane 1: MWM, Lane 2: Mouse Heart Tissue Extract, Lane 3: Chicken Heart Tissue Extract.